Year: 2017 | Month: April | Volume 7 | Issue 1
Effect of Low dose Chronic Exposure to Deltamethrin and Lindane on Reproductive System of Male Mice
Abstract:
In the present study, 198 Swiss albino male mice were exposed to daily oral doses of deltamethrin and lindane to study the effect of low dose chronic exposure to reproductive organs of male mice. Mice were exposed for 15, 30 and 60 days Doses of pesticides were selected on the basis of maximum residual limits (MRL) as well as the residues of pesticides found in various food commodities in the previous studies. Thus the three doses used in the study were 0.5, 1.0 and 1.5 mg/kg bwt/day for deltamethrin and 0.25, 0.5 and 0.75 mg/kg bwt/day for lindane. The highest dose of deltamethrin and lindane used in this study was 1/40th and 1/200th of the LD50 of these two pesticides, respectively. After the completion of experimental trial, the mice were sacrificed and testicular tissues were collected and preserved in neutral buffered formalin till analysis. The sections of tissues (testes) were taken and stained with haematoxylin and eosin to visualize the histopathological changes caused due to exposure of mice to the pesticides. The histological examination revealed the degenerative changes i.e. vacuolar degeneration of the spermatogenic cells, presence of necrotic cells and edema inside the seminiferous tubule and aspermatogenesis at all the dose and duration of exposure to each of the pesticide. The changes were more or less dependent upon the dose of pesticide as well as the duration of exposure to these pesticides. This was further confirmed by the residues of pesticides detected in these vital tissues through gas chromatography (GC) and confirmation with gas chromatography-mass spectrophotometry (GC-MS).
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